| Intra-peritoneal NF-kappaB decoy oligodeoxynucleotide decreases postoperative intra-abdominal adhesion. |
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Byul Nim Ahn, Mi Hyun Song, Ju Hyun Kim, Kyung Hyun Kim, Kwan Kyu Park, Youn Seok Choi |
1Department of Obstetrics and Gynecology, Catholic University of Daegu School of Medicine, Daegu, Korea. drcys@cu.ac.kr
2Department of Pathology, Catholic University of Daegu School of Medicine, Daegu, Korea. |
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| Abstract |
OBJECTIVE
The objective of this study was to evaluate the effect of intra-peritoneal nuclear factor kappaB (NF-kappaB) decoy oligodeoxynucleotide on the prevention of postoperative intra-abdominal adhesion. METHODS: Forty female BALB/c mice were randomly assigned into 2 groups. The mice were anesthetized and then midline abdominal incision was made. Peritoneal injury was given using cytobrush until deperitonealization. The experimental group (n = 20) received NF-kappaB decoy oligodeoxynucleotide (NF-kappaB decoy ODN) in the peritoneal cavity before abdominal closure, while the control group (n = 20) received saline. At 2 weeks postoperation, after the mice were euthanized, the status of intra-abdominal adhesion was evaluated. The degrees of adhesion were graded into no (grade 0), mild (grade 1), moderate (grade 2), and severe adhesion (grade 3). RESULTS: Two mice in the experimental group and 3 mice in the control group were dead within 1 day postoperation. In the control group, 2, 2, and 13 mice showed mild, moderate, and severe adhesion, respectively. In the experimental group, 4 mice showed no adhesion, whereas, 6 and 8 mice showed mild and moderate adhesion, respectively. The difference of degrees of adhesion between the groups was statistically significant (P <0.001). CONCLUSION: Intra-peritoneal NF-kappaB decoy ODN reduced the severity of postoperative intra-abdominal adhesion. |
| Key Words:
Adhesion, Inflammation, NF-kappa B, NF-kappa B decoy oligodeoxynucleotide |
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