Korean J Obstet Gynecol Search


Korean Journal of Obstetrics & Gynecology 1997;40(5):941-946.
Published online January 1, 2001.
The Effects of Free Radical Scavenger, Rutin, on the Development and the Cell Number of Blastocyst in Mouse Early Embryos.
Kyung Joo Hwang, Hyuck Chan Kwon, Hee Sug Ryu, Haeng Soo Kim, Hyun Won Yang, Kie Suk Oh
Department of Obstetrics and Gynecology, Ajou University School of Medicine, Swon, Korea.
It is well known that developmental delay or arrest occurs before implantation inmammals, which have undergone in vitro culture. Recently, these phenomenon are being attributedto oxygen free radicals, and successful cell culture are being obtained by lowering theoxygen environment of in vitro culture. This is due to the fact that the oxygen concentrationin the fallopian tube is around 5%, which is lower than the room air 20% concentrationfor in vitro culture.Rutin, which is a free radical scavenger, was added to early embryo mice culture andcompared the free radical level at blastocyst stage with that of different culture conditions,and found that free radical level was markedly decreased. Also, there was increased embryodevelopment with decreasing free radical levels in the experimental group, and there wassignificant increase in the blastulation rate and blastomere count.This study therefore suggests the possibility of improved in in-vitro embryo culture.
Key Words: Oxygen free radicals, Rutin, Free radical scavenger, Blastulation rate, Blastomere count

Article category

Browse all articles >


Browse all articles >

Editorial Office
4th Floor, 36 Gangnam-daero 132-gil, Gangnam-gu, Seoul 06044, Korea.
Tel: +82-2-2266-7238    Fax: +82-2-3445-2440    E-mail: journal@ogscience.org                

Copyright © 2024 by Korean Society of Obstetrics and Gynecology.

Developed in M2PI

Close layer
prev next