Korean J Obstet Gynecol Search


Korean Journal of Obstetrics & Gynecology 1997;40(9):2008-2014.
Published online January 1, 2001.
The Expression of TGF-beta1 and Its Effects on Collagen I and III Messenger Ribonucleic Acid Levels in Uterine Leiomyomas and Myometriums.
Byung Seok Lee, Ki Hyun Park, Dong Jae Cho, Kook Lee, Jae Wook Kim, Chan Ho Song, Woo Ickz Yang
1Department of Obstetrics and Gynecology, College of Medicine, Yonsei University, Korea.
2Department of Pathology, College of Medicine, Yonsei University, Korea.
Leiomyomas, which are the commonest pelvic tumors in women, are originated from myometrial cells. Although the exact initial pathophysiologic event of the leiomyoma is not known, recent evidences suggested that the effects of sex steroid hormones in the process of tumor growth are mediated by local production of growth factors including epidermal growth factor(EGF), insulin-like growth factor-I(IGF-I) and insulin-like growth factor-II(IGF-II). Recently, it was suggested that transforming growth factor-beta(TGF-beta) may play a role for growing of leiomyomas. It was known that TGF-beta increase the formation of extracellular matrix. And we examined TGF-beta1 expression and tried to know the effect of TGF-beta1 on the extracellular matrix, collagen I and III mRNA levels in cultured leiomyoma and myometrial cells. We found that immunoreactive TGF-beta1 was expressed in myometriums and in leiomyomas. There was no difference at the intensity of immunostaining between two tissues. In northern blotting, there was no difference of TGF-beta1 expression at the transcriptional level in both tissues. TGF-beta1 increased the mRNA levels of collagen I in cultured myometrial and leiomyoma cells at the concentration of 1 ng/ml(p<0.05), 10 ng/ml(p<0.001). And TGF-beta1 increased the mRNA levels of collagen III in cultured myometrial and leiomyoma cells at the concentration of 1 ng/ml (p<0.05), 10 ng/ml(p<0.05). Therefore, expressed TGF-beta1 may stimulate the incorporation of collagen I and III into the extracellular matrix in myometriums and leiomyomas.
Key Words: Myometrium, Leiomyoma, Transforming growth factor-beta, Collagen

Article category

Browse all articles >


Browse all articles >

Editorial Office
4th Floor, 36 Gangnam-daero 132-gil, Gangnam-gu, Seoul 06044, Korea.
Tel: +82-2-2266-7238    Fax: +82-2-3445-2440    E-mail: journal@ogscience.org                

Copyright © 2024 by Korean Society of Obstetrics and Gynecology.

Developed in M2PI

Close layer
prev next