Study on the Detection of Chromosome X, Y Using in situ PCR.

Kim, Young Ju

Original Article

Korean Journal of Obstetrics & Gynecology 1997;40(11):2568-2572.
Published online January 1, 2001.

Study on the Detection of Chromosome X, Y Using in situ PCR.

Department of Obstetrics and Gynecology, Devision of Tissue and Cell Biology, Ewha Medical Research Center, College of Medicine, Ewha Womans' University, Korea.

Abstract

Untill now, classical karyotyping remains the most reliable and conclusive method, but sometimes very rapid and sensitive method for the detection of chromosomal abnormality is needed. The primed in situ labelling(PRINS) is very rapid and sensitive method for the detection of chromosomal aneuploidy and X-linked disorder instead of fluorescen in situ hybridization. The purpose of this study was to prepent the application of PRINS protocol to the rapid detection of chromosomes X and Y in both metaphases and interphase nuclei. The PRINS reaction was done in 10 amniocytes. We compared the PRINS result with conventional cytogenetic karyotyping. The result was the same with cytogenetic karyotyping. In the future, PRINS will become very useful and sensitive method for the chromosomal aneuploidy detection and X-linked disorder diagnosis.

Key Words: In situ PCR, PRINS, Chromosome, Prenatal diagnosis