Korean J Obstet Gynecol Search

CLOSE


Korean Journal of Obstetrics & Gynecology 1998;41(5):1315-1322.
Published online January 1, 2001.
Rapid Prenatal Diagnosis of Chromosomal Aneuploidies by Using Fluorescence in Situ Hybridization ( FISH ).
I K Kim, Y H Yang, E K Choi, M S Kim, J Y Kim
Abstract
INTRODUCTION: The traditional cytogenetic analysis requires relatively long cell culture time, intensive labour and trained personnel. But in clinical situations, the prenatal diagnosis of genetic disease and incidental finding of abnormal ultrasonographic finding need urgent decisions on future management. So we need more rapid and precise diagnostic tools of prenatal genetic counselling. The fluorescence in situ hybridization (FISH) has been studied for detecting chromosomal aneuploidies because this method can get rapid results of cytogenetic studies. OBJECTIVES: To evaluate the clinical utility of prenatal diagnosis of chromosomal aneuploidies by using FISH. Materials and methods: chorionic villi (n=6), amniotic fluid (n=7), fetal blood (n=2) were obtained from 15 pregnancies undergoing fetal karyotyping at 9 to 30 weeks of gestation for prenatal genetic counselling.Karyotyping was performed by both traditional cytogenetics and FISH, using commertially available kits. After the procedures, the results of FISH were compared with the results of traditional cytogenetic studies. RESULTS: In a blind series of 15 samples all, including one case of trisomy 21, one case of trisomy 18 and one case of monosomy X, were correctly identified. CONCLUSIONS: FISH is a rapid and effective methods for detection of chromosomal aneuploidies in prenatal diagnosis and yields possible clue of fetal genetic diagnosis with pregnant maternal peripheral blood.
Key Words: Rapid prenatal diagnosis, Fluorescence in situ hybridization, Chromosomal aneuploidies


ABOUT
ARTICLE & TOPICS
Article category

Browse all articles >

Topics

Browse all articles >

BROWSE ARTICLES
POLICY
FOR CONTRIBUTORS
Editorial Office
4th Floor, 36 Gangnam-daero 132-gil, Gangnam-gu, Seoul 06044, Korea
Tel: +82-2-3445-2382    Fax: +82-2-3445-2440    E-mail: journal@ogscience.org                

Copyright © 2021 by Korean Society of Obstetrics and Gynecology. All rights reserved.

Developed in M2PI

Close layer
prev next