Korean J Obstet Gynecol Search

CLOSE


Korean Journal of Obstetrics & Gynecology 1999;42(5):1024-1029.
Published online January 1, 2001.
Regulation of Oxytocin Receptor by Interleukin-6 on Cultured Myometrial Cells.
K H Lee, Y S Park, Y S Shin, J Kim, K C kang
Abstract
OBJECTIVE
Inflammatory cytokines seem to play a key role in mechanisms initiating labor. The objective of this study was to assess the effects of interleukin[Il]-6 on expression of human myometrial oxytocin receptors. METHODS: We prepared a human myometrial cell culture system. Myometrial cells collected from 12 patients were treated with Il-6 of different concentrations[10-7, 10-9, 10-12M] for 3 days and oxytocin receptor protein was determined by Western blotting. To demonstrate oxytocin receptor messenger RNA, reverse transcription-polymerase chain reaction was performed. RESULTS: Western blotting showed a single 70-kd band corresponding to the oxytocin receptor. Treatment with higher concentration[10-7, 10-9M] of Il-6 led to a significant increase in myometrial oxytocin receptor protein levels[p=0.001, 0.002 respectively]. However, treatment with lower concentration[10-12M] did not increase oxytocin receptor levels[p=0.1]. Reverse transcription-polymerase chain reaction showed oxytocin receptor messenger ribonucleic acid as a 391-bp band. CONCLUSION: In cultured myometrial cells, Il-6 increase significantly oxytocin receptor protein level. This study has added further support to the notion that the myometrium serves as a target for the inflammatory cytokine, Il-6, thus promoting preterm labor associated with intrauterine infection.
Key Words: Oxytocin receptor, Il-6, Myometrium, Culture
TOOLS
Share :
Facebook Twitter Linked In Google+ Line it
METRICS Graph View
  • 742 View
  • 10 Download
Related articles in Obstet Gynecol Sci

The Regulation of Cyclooxygenase-2 Expressionby Interleukin-1beta in WISH cells.2001 August;44(8)



ABOUT
ARTICLE & TOPICS
Article category

Browse all articles >

Topics

Browse all articles >

BROWSE ARTICLES
POLICY
FOR CONTRIBUTORS
Editorial Office
4th Floor, 36 Gangnam-daero 132-gil, Gangnam-gu, Seoul 06044, Korea.
Tel: +82-2-2266-7238    Fax: +82-2-3445-2440    E-mail: journal@ogscience.org                

Copyright © 2024 by Korean Society of Obstetrics and Gynecology.

Developed in M2PI

Close layer
prev next