Role of annexin-I in cervical cancer cell proliferation.

Lew, Young Ok; Park, Tae Chul; NamKoong, Sung Eun; Park, Dong Choon; Kim, Jae Hoon; Kim, Dae Hoon; Yang, Yong Jae; Lee, Jee Hyun; Cho, Sung Dae; Jo, Hyeong Kwon

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Original Article

Korean Journal of Obstetrics & Gynecology 1999;42(10):2199-2204.
Published online January 1, 2001.

Role of annexin-I in cervical cancer cell proliferation.

Young Ok Lew, Tae Chul Park, Sung Eun NamKoong, Dong Choon Park, Jae Hoon Kim, Dae Hoon Kim, Yong Jae Yang, Jee Hyun Lee, Sung Dae Cho, Hyeong Kwon Jo

Abstract

OBJECTIVES
To investigate the role of annexin-I in human cervical cancer, we evaluated the expression of annexin-I and the relation with the proliferation of cancer cells. Methods: By immunohistochemical analysis and the western blotting of annexin-I , we investigated the extent and distribution of the expression of annexin-I in cervical cancer tissues. After treating the human cancer cell lines ( SiHa and HeLa cell lines ) with tamoxifen, estradiol, and retinoic acid for 5 days to make the cells proliferate and antiproliferate, we measured the proliferation simultaneously with 3-(4,5- dimethyl thiazol- 2-yl)-2,5-diphenyl-tetr -azolium bromide (MTT) colorimetric assay and the expression level of annexin-I with flowcytometry. RESULTS: In the immunohistochemical stains, a granular staining pattern involving the entire cytoplasm was more heavily observed in malignant lesions than in normals. In the western blotting, the antibodies against 35-kDa annexin-I appeared to react more strongly with the lysates of cancer tissues than normal and benign tissues. In SiHa and HeLa cell lines with tamoxifen and beta- estradiol treatment, increased expressions of annexin-I were noted with correlated increased proliferation of cells, and with the treatments of all trans retinoic acid, decreased expressions of annexin-I were noted with correlated decreased proliferation of cells. CONCLUSIONS: The results suggest that the expression of annexin-I might correlate with cervical cancer than normal and the proliferation of cancer cells.

Key Words: Annexin-I, Cervical cancer, Proliferation

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