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Korean Journal of Obstetrics & Gynecology 2001;44(4):748-754.
Published online April 1, 2001.
Regulatory Mechanism of COX-2 by Estrogen and Progesterone in Uterine Cell Lines of Rodents.
Byung Moon Kang, Eun Ju Park, Sung Hoon Kim, Dae Joon Cheon, Hee Dong Chae, Chung Hoon Kim, Kyun Park, Yoon Seok Chang
1Department of Obstetrics and Gynecology, College of Medicine, University of Ulsan, Asan Medical Center, Seoul, Korea.
2Department of Obstetrics and Gynecology, College of Medicine, University of Ulsan, Ulsan University Hospital, Ulsan, Korea.
Abstract
OBJECTIVE
To evaluate the regulatory mechanism of cyclooxygenase(COX)-2 by estrogen and progesterone in uterine cell lines of rodents MATERIALS AND METHODS: CUE-P cells, originated from the uterine endometrial epithelial cells, and CUE-V2 cells, originated from the uterine endometrial stromal cells, were incubated in the Dulbecco's modified Eagle's medium(DMEM) and F12 medium. The cellular proliferation were evaluated every 24 hours. The expression of COX-2 was measured after estrogen and progesterone supplementation by western blot. RESULTS: CUE-P cells have cuboidal morphology and CUS-V2 cells resemble fibroblast and exhibit a spindle-like morphology. When CUE-P and CUS-V2 cells were co-cultured with estrogen and progesterone supplementation, the expression of COX-2 was decreased after 24 hours of estrogen supplementation. The decreased expression of COX-2 was observed only when estrogen was supplemented after 9 hours of initial progesterone supplementation. CONCLUSION: The sex steroid, such as estrogen and progesterone could regulate COX-2 expression in uterine cell lines.
Key Words: cyclooxygenase-2, CUE-P, CUE-V2, estrogen, progesterone, expression
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