| Effect of superovulation on the expression of Tissue Inhibitor of Metalloproteinases-3 and Matrix Metalloproteinases-9 in murine endometrium. |
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Seung Yup Ku, Young Min Choi, Yon Su Kim, Jin Yong Lee |
1Department of Obstetrics and Gynecology, College of Medicine.
2Institute of Reproductive Medicine and Population, Medical Research Center.
3Department of Internal Medicine, College of Medicine, Seoul National University, Seoul, Korea. |
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| Abstract |
OBJECTIVES
The effects of superovulation on the expression of mRNA and protein of TIMP-3 and MMP-9 in murine endometrium were assessed. METHODS: Using murine pregnant uteri of gestation day (g.d.) 4, 5 and 6 after injection of PMSG 5 and 10 IU, the effects of superovulation were assessed and compared with those of natural pregnancy and pseudopregnancy groups using quantitative competitive RT-PCR and immunohistochemical staining. RESULTS: Expression of TIMP-3 mRNA and protein showed an increase in PMSG groups and pseudopregnancy group, while there was no difference in MMP-9 expression between natural pregnancy and PMSG, pseudopregnancy groups on g.d. 4 through g.d. 6. CONCLUSIONS: This study suggests that ovarian hyperstimulation by gonadotropin, which produces many oocytes and embryos, may have a detrimental effect on embryonic implantation and its relevant endometrial remodeling process by increase in expression of TIMP-3 in murine endometrium. |
| Key Words:
Superovulation, TIMP-3, MMP-9, Endometrium, Mouse |
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