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Korean Journal of Obstetrics & Gynecology 2001;44(6):1056-1061.
Published online June 1, 2001.
Histologic Diagnosis of Cervcial Precursor Lesion: Applying the Bethesda System and HPV 16, 18 Correlates.
Jong Lee, Mi Sook Kim, Chi Dong Han, Jeong Ja Park, Jae Bok Park, Tae Sung Lee
1Department of Obstetrics and Gnecology, College of Medicine, Catholic University of Daegu.
2Department of Pathology, College of Medicine, Catholic University of Daegu.
Abstract
OBJECT: To support the application of the Bethesda classification to the histologic diagnosis of cervical squamous lesions. METHODS: We evaluated the cervical biopsies and human papilloma virus (HPV) DNA status of 100 clinic patients. The 100 biopsy specimens were analyzed using criteria of the Bethesda system for low-grade squamous intraepithelial lesion(LSIL) and high-grade squamous intraepithelial lesions(HSIL) in association with analysis of HPV DNA virus type by PCR. RESULTS: By PCR amplified from paraffin embedded tissues, the 40 of 90 cases of classified as HSIL were HPV 16 or 18 positive and 1/10 cases of classified as LSIL was HPV 16 positive. CONCLUSION: This study supports the usefullness of certain histologic criteria of the Bethesda system for distinguishing squamous intraepithelial lesions into two grades.
Key Words: Cervix, Human papillomavirus, PCR, The Bethesda System
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