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Korean Journal of Obstetrics & Gynecology 2001;44(8):1507-1513.
Published online August 1, 2001.
Influence of Hepatocyte Growth Factor on its Receptor (c-met) expression and Invasion in HT Cell Line.
Jong Chul Shin, Jee Hyun Lee, Dong Eun Yang, Min Jeong Oh, Hee Bong Moon, Young Lee, Sa Jin Kim, Chong Seung Yi, Soo Pyung Kim
Department of Obstetrics and Gynecology, Catholic University Medical College, Seoul, Korea.
Abstract
OBJECTIVE
To evaluate the invasive capacity of trophoblastic cells when treated with HGF, and see whether HGF induces c-met expression in vitro. METHODS: HT cell line was treated with recombinant HGF (rHGF) at different concentrations (0, 10, 50 and 100 ng/mL) and cultured for 24 hours. To evaluate according different time of culture, HT cell line was pre-treated with 50 ng/mL rHGF and cultured for 24, 36, 48 and 72 hours. We analyzed the expression of c-met mRNA using reverse transcriptase-polymerase chain reaction and the expression of c-met protein using western blot in each samples. We also observed cellular invasion capacity through the invasion assay under a microscope and confirmed 72 kDa gelatinase and 92 kDa gelatinase expression patterns by zymography assay. RESULTS: The expressions of c-met mRNA and protein were increased in all concentrations of rHGF, compared with that of normal control although it was not in dose-dependent fashion. In invasion assay, the number of invaded HT cells were increased in dose-dependent fashion, compared with that of normal control. In zymography ssay, the expression of 72 kDa gelatinase was increased in dose-dependent fashion, compared with the control. However, 92 kDa eglatinase was not detected in any studied group. CONCLUSION: These results suggests that HGF might be related to upregulation of trophoblast cell invasiveness by activation of c-met and subsequent induction of 72 kDa gelatinase.
Key Words: Trophoblast, HGF, c-met, Invasiveness


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