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Korean Journal of Obstetrics & Gynecology 2001;44(9):1602-1608.
Published online September 1, 2001.
Damage of Intracytoplasmic Meiotic Spindles in Cryopreserved-Thawed Mouse Oocytes.
Seok Hyun Kim, Sung Mi Choi, Byung Chul Jee, Hee Sun Kim, Sun Kyung Oh, Chang Suk Suh, Young Min Choi, Jung Gu Kim, Jin Yong Lee, Shin Yong Moon
1Department of Obstetrics and Gynecology, College of Medicine, Seoul National University, Seoul, Korea.
2Institute of Reproductive Medicine and Population, Medical Research Center, Seoul National University, Seoul, Korea.
Abstract
OBJECTIVE
To investigate the extent of meiotic spindle damages in cryopreserved-thawed mouse mature oocytes. METHODS: After slow freezing and ultra-rapid thawing using 1.5M dimethylsulfoxide(DMSO), mouse mature oocytes were stained by anti-alpha tubulin monoclonal antibody. The meiotic spindle and chromosomes configuration were assessed using confocal microscope. The influence of time to post-hCG oocytes retrieval (i.e. 12 hrs vs 17 hrs) was also evaluated. RESULTS: The normal meiotic spindles were observed in 89.8% of post-hCG 12 hrs group, and 80.1% of post-hCG 17 hrs group, and these were significantly lower than that of each unfreezed control. Post-hCG 12 hrs group showed a significantly higher incidence of normal meiotic spindles, compared with post-hCG 17 hrs group. CONCLUSION: The extent of meiotic spindle damages was significantly increased after cryopreservation in mouse mature oocytes. We proposed that 12 hrs interval of post-hCG oocytes retrieval may be more beneficial.
Key Words: Mouse oocytes, Cryopreservation, DMSO, Slow freezing and ultrarapid thawing, Meiotic spindle
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