| Damage of Intracytoplasmic Meiotic Spindles in Cryopreserved-Thawed Mouse Oocytes. |
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Seok Hyun Kim, Sung Mi Choi, Byung Chul Jee, Hee Sun Kim, Sun Kyung Oh, Chang Suk Suh, Young Min Choi, Jung Gu Kim, Jin Yong Lee, Shin Yong Moon |
1Department of Obstetrics and Gynecology, College of Medicine, Seoul National University, Seoul, Korea.
2Institute of Reproductive Medicine and Population, Medical Research Center, Seoul National University, Seoul, Korea. |
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| Abstract |
OBJECTIVE
To investigate the extent of meiotic spindle damages in cryopreserved-thawed mouse mature oocytes. METHODS: After slow freezing and ultra-rapid thawing using 1.5M dimethylsulfoxide(DMSO), mouse mature oocytes were stained by anti-alpha tubulin monoclonal antibody. The meiotic spindle and chromosomes configuration were assessed using confocal microscope. The influence of time to post-hCG oocytes retrieval (i.e. 12 hrs vs 17 hrs) was also evaluated. RESULTS: The normal meiotic spindles were observed in 89.8% of post-hCG 12 hrs group, and 80.1% of post-hCG 17 hrs group, and these were significantly lower than that of each unfreezed control. Post-hCG 12 hrs group showed a significantly higher incidence of normal meiotic spindles, compared with post-hCG 17 hrs group. CONCLUSION: The extent of meiotic spindle damages was significantly increased after cryopreservation in mouse mature oocytes. We proposed that 12 hrs interval of post-hCG oocytes retrieval may be more beneficial. |
| Key Words:
Mouse oocytes, Cryopreservation, DMSO, Slow freezing and ultrarapid thawing, Meiotic spindle |
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