| A New Prenatal Diagnosis of Fetal Cells Isolation from Maternal Peripheral Blood -Using Comparative Genomic Hybridization by Microdissection. |
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Young Ho Yang, Sung Hoon Kim, Sei Kwang Kim, Yong Won Park, Jae Sung Cho, In Kyu Kim, Jong Rak Choi, Mi Soon Kim |
1Department of Obstetrics and Gynecology, College of Medicine, Yonsei University, Seoul, Korea.
2Department of Clinical Pathology, College of Medicine, Yonsei University, Seoul, Korea.
3Division of Prenatal Genetic Clinic, College of Medicine, Yonsei University, Seoul, Korea.
4The Genetic Laboratory of the Medical Research Center, College of Medicine, Yonsei University, Seoul, Korea. |
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| Abstract |
OBJECTIVE
The objective of this study was to determine the clinical use of CGH (comparative genomic hybridization) for detection of fetal aneuploidy from fetal cells (nucleated red blood cells, nRBCs) isolated from maternal peripheral blood. METHODS: Maternal peripheral venous blood sample was collected and treated by heparin. Triple density gradient centrifugation, and MACS (magnetic activated cell sorting) using CD45 and CD 71 were used to isolated the fetal nRBCs. With microdissection, DOP (degenerate oligonucleotide primed)-PCR (polymerase chain reaction), and nick translation, CGH was performed. RESULTS: Fetal nRBCs were successfully isolated from maternal peripheral blood. After microdissection of fetal nRBCs, DOP-PCR. and nick translation, DNA size was suitable for hybridization. In CGH analysis, we can confirm normal female and trisomy 21 male fetus. CONCLUSION: Prenatal diagnosis from fetal cells in maternal peripheral blood by comparative genomic hybridization shows clinical promise in terms of speed, accuracy, and non-invasiveness. To enable widespread use of this method, further studies involving many cases are warrented. |
| Key Words:
Prenatal diagnosis, Fetal nRBCs, Microdissection, Comparative genomic hybridization |
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