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Korean Journal of Obstetrics & Gynecology 2002;45(7):1191-1203.
Published online July 1, 2002.
The Gene Expression Profile Using cDNA microarray after treatment Arsenic Compound (As2O3, As4O6) in SiHa Cell.
Young Mi Seo, Kyung Eun Park, Young Gi Pang, Kook Chong Kim, Serk Yong Park, Woong Shick Ahn, Jun Mo Lee, Sung Eun Namkoong, Do Gang Kim
1Department of Obstetrics and Gynecology, College of Medicine, The Catholic University of Korea.
2Department of Obstetrics and Gynecology, College of Pharmacy, Seoul National University.
3Department of Medical Technology, College of Health Science, Younsei University.
Abstract
OBJECTIVE
To obtain information on the growth inhibition effect of arsenic compounds and gene expression profiles using cDNA microarray technique in SiHa cell lines. METHODS: We cultured 103 SiHa cell in 96 well plate and we investigated growth inhibition effects using MTT assay and also we performed gene expression profile experiment using 384 cDNA chip in SiHa cell after exposure of arsenics (As2O3, As4O6 - 1 (micro)M) for 48 hrs. RESULTS: Arsenics (As2O3, As4O6) inhibit the growth of SiHa cells (As2O3: 0.5, 1, 2, 3, 4, 5 (micro)M - 9.2, 56, 89, 93, 96, 96%, As4O6: 0.5, 1, 2, 3, 4, 5 (micro)M- 54, 84, 84, 85, 85, 87%) in 4 days culture. As2O3 and As4O6 induced apoptosis in SiHa cells. After exposure of As2O3, 47 genes were changed more than 2 times (eg, thymidylate synthetase, cyclin B1, CDC 20). In case of As4O6, 78 genes were changed more than 2 times (eg, CDC 20, cyclin B1, primase, proliferating cell nuclear antigen). CONCLUSION: we observed arsenic compound (As2O3, As4O6) inhibit the growth of SiHa cell. In gene expression profiling experiment, 78 genes was changed the expression level 2 times more than that of reference RNA after treatment of As4O6 and 47 genes after treatment of As2O3. Through these result, we thought more study need in functional genomics after arsenic treated cervical cancer cells.
Key Words: Arsenic compound (As2O3, As4O6), Cervical cancer cell line (SiHa), cDNA chip
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