Expression and Localization of the PlGF mRNA in Preeclamptic Placentas.

Lee, Soon Ae; Noh, Gu Seop; Choi, Won Joon; Lee, Jong Hak; Paik, Won Young

Obstet Gynecol Sci > Volume 45(10); 2002 > Article

Original Article

Korean Journal of Obstetrics & Gynecology 2002;45(10):1778-1784.
Published online: October 1, 2002

Expression and Localization of the PlGF mRNA in Preeclamptic Placentas.

Soon Ae Lee, Gu Seop Noh, Won Joon Choi, Jong Hak Lee, Won Young Paik

¹Department of Obstetrics and Gynecology, GyeongSang National University, College of Medicine Chinju, Korea.
²Department of Anatomy, GyeongSang National University, College of Medicine Chinju, Korea.

Abstract

OBJECTIVE
To identify and localize the Placenta Growth Factor (PlGF) mRNA in term placentas and to determine whether there are any differences in the expression of PlGF mRNA in the placentas between normal and preeclamptic pregnancy. METHODS: Northern blot and In situ hybridization with 5 cases of preeclamptic placentas and 8 cases of normal controls RESULTS: In Northern blot analysis and In situ hybridization, PlGF2 mRNA (1.7 kb) band was identified and showed significant decrease in density in preeclamptic placentas compared to that of normal controls. PlGF mRNA was mainly expressed in the syncytial membrane of villous trophoblast, and in the perivascular tissue surrounding the vasculature of stem villi. CONCLUSION: Decreased levels of PlGF mRNA in preeclamptic placentas could have some unfavorable influences on trophoblast and endothelial function, thereby may play a role to the pathogenesis of preeclampsia.

Keywords: Preeclampsia; PlGF mRNA; Placenta