| Comparative Analysis of Multiplex PCR and Hybrid Capture System in the Detecttion of Human Papillomavirus in the Uterine Cervix. |
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Sei Jun Han, San Kim, Min Chang Kang, Ju Seong Lim, Tae Gyu Ahn, Byung Rai Lee |
1Department of Obstetrics and Gynecology, College of Medicine, Chosun University, Gwangju, Korea.
2Department of Biochemistry, College of Medicine, Chosun University, Gwangju, Korea. |
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| Abstract |
OBJECTIVE
To compare the multiplex-PCR and hybrid capture methods for detection of Human papillomavirus (HPV) in uterine cervical swab samples. METHODS: This study determined the HPV infection and its risk group (subtype) in uterine cervical swab samples of 91 Korean women by HPV hybrid capture I and multiplex-PCR method. RESULTS: The prevalence of HPV infection determined by muliplex-PCR was more higher than by hybrid capture method I. Of 65 women with nonspecific uterine cervical lesions, the frequence of HPV-positivity was 14/65 (12 low-risk and 2 high-risk HPV) by hybrid capture method I and was 37/65 (34 low-risk and 3 high- risk HPV) by multiplex-PCR method, respectively. The frequence of high-risk HPV was 2/6 in LSIL, 7/14 in HSIL, 4/6 in uterine cervical cancer by hybrid capture method I, and was 2/6 in LSIL, 8/14 in HSIL, 4/6 in uterine cervical cancer by multiplex-PCR method. CONCLUSION: These results suggest that multiplex-PCR is more sensitive, simple and cost-effective technique than Hybrid-capture I in the detection of HPV infection in the uterine cervix. |
| Key Words:
HPV, Multiplex PCR, Hybrid capture I |
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