| Chromosomal gains and losses in primary ovarian carcinomas by comparative genomic hybridization. |
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Soo Hun Cho, Mee Hye Kim, Nak Woo Lee, Young Tae Kim, Kyu Wan Lee |
1Department of Obstetrics and Gynecology, College of Medicine, Korea University, Korea.
2Sewha Pediatric Clinic, Seoul, Korea. |
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| Abstract |
OBJECTIVE
Comparative genomic hybridization was performed to evaluate DNA sequence copy number changes in human ovarian carcinomas from paraffin-embedded tissue blocks. PATIENTS AND METHODS: DNA from 20 cases of primary ovarian carcinomas underwent comparative genomic hybridization to evaluate the extent of genetic gains or losses in a test sample. RESULTS: In thirteen cases of 20 samples, varying degree of genetic imbalances was observed. Of the remaining 7 cases, two revealed normal, five failed to yield a result. Most common genetic imbalances are 8q22.2-q24 site amplification and 12p site amplification, where c-myc gene and k-ras gene respectively are included. Second most common site of genetic imbalance is 7p21-pter site deletion. CONCLUSION: Our results have shown many chromosomal alterations in human ovarian carcinomas, and these sites are known previously as oncogene or tumor-suppression gene, and some sites are not known specific cancer associated sites. Our data can be useful for screening chromosomal changes and molecular mechanism of human ovarian carcinogenesis. |
| Key Words:
ovarian cancer, comparative genomic hybridization, FISH, chromosomal change |
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