Prevalence and Genotyping of HPV in Cervical Cancer of Korean Women.

Kim, Yong Beom; Jeon, Yong Tark; Seo, Sang Soo; Kim, Jae Weon; Park, Noh Hyun; Song, Yong Sang; Kang, Soon Beom; Lee, Hyo Pyo

Original Article

Korean Journal of Obstetrics & Gynecology 2003;46(4):789-794.
Published online April 1, 2003.

Prevalence and Genotyping of HPV in Cervical Cancer of Korean Women.

Yong Beom Kim, Yong Tark Jeon, Sang Soo Seo, Jae Weon Kim, Noh Hyun Park, Yong Sang Song, Soon Beom Kang, Hyo Pyo Lee

Department of Obstetrics and Gynecology, College of Medicine, Seoul National University, Seoul, Korea.

Abstract

OBJECTIVE
To evaluate the prevalence and genotype of high risk HPV infection with oligonucleotide microarray based DNA chip in cervical cancer. METHODS: The presence of HPV and its type were tested with oligonucleotide microarray based DNA chip in 84 specimen from cervical cancer (squamous cell carcinoma; 58, adenocarcinoma; 22, adenosquamous carcinoma and small cell carcinoma; 4). RESULTS: The overall detection rate of high risk HPV infection was 84.5% (71/84). In squamous cell carcinoma and adenocarcinoma, the detection rate were 79.3% (46/58) and 100% (22/22) respectively. The distribution of HPV types in squamous cell carcinoma and adenocarcinoma was as follows; HPV 16 (60.3%, 59.1%), HPV 18 (1.7%, 27.3%). CONCLUSION: The most common type of high risk HPV detected in squamous cell carcinoma and adenocarcinoma of cervix was HPV 16 followed by HPV 18. In contrast with squamous cell carcinoma, HPV 18 was more frequently detected than HPV 16 in adenocarcinoma. HPV DNA chip seems to be effective in the detection and typing of HPV in tissues from cervical cancer.

Key Words: Cervical cancer, Human papilloma virus, DNA chip, Prevalence, Genotype