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Korean Journal of Obstetrics & Gynecology 2003;46(8):1500-1509.
Published online August 1, 2003.
The Modulation of IL-12 by Phosphodiesterase IV Inhibitor and Subsequent Changes of Th-1, Th-2 Cytokines in Human Endometrial Tissue.
Won Il Park, Eun Kyong Kim, Duck Sung Ko, Seo You Hong, Joong Yol Na, Jung Hwan Shin, Dae Woon Kim
1Department of Obstetrics and Gynecology, College of Medicine, Eulji University, Korea.
2Department of Pathology, College of Medicine, Eulji University, Korea.
3Life Science Institute School of Medicine, Eulji University, Korea.
4Department of Obstetrics and Gynecology, College of Medicine, Korea University, Korea.
Abstract
OBJECTIVE
To assess the capability of phosphodiesterase type IV inhibitor (rolipram) to suppress IL-12 in human decidua and the subsequent changes of Th-2 cytokine (IL-10) and Th-1 cytokine (TNF-alpha). METHODS: Decidual tissues of 10 first-trimester pregnant women and 10 first-trimester pregnant women diagnosed as missed abortion were collected by dilatation and currettage. The decidual tissues were treated with rolipram for 6 hours. Protein and mRNA expression in the tissues were analysed by western blotting, immunohistochemistry and reverse transcription-polymerase chain reaction. RESULTS: Rolipram, in the concentration above 1 microgram/ml, could decrease the expression of IL-12p35 (control: 46.37+/-7.38, rolipram: 24.34+/-8.46) and IL-12p40 mRNA (control: 31.7+/-5.8, rolipram: 14.9+/-4.6) and protein (control: 52.4+/-8.9, rolipram: 40.9+/-12.1). However, the expression of IL-10 and TNF-alpha mRNA and protein did not changed by rolipram. There was no difference in the cytokine expression pattern between the decidual tissues of normal pregnancy and missed abortion. CONCLUSION: Rolipram, the phosphodiesterase type IV inhibitor, could induce the decrease of IL-12 in human decidua. In human decidual tissue, unlike other human tissues, the decrease of IL-12 by rolipram did not modulate other Th-1/Th-2 cytokines. Inability of IL-12 to modulate other Th-1/Th-2 cytokines might be related with unique cytokine network in human decidua rather than its small extent of decrease.
Key Words: Rolipram, Decidua, IL-12, Th1 cytokine, Th2 cytokine
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