| Activation of Nuclear Factor Kappa B by Inducers in Gestational Tissues at Term. |
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Jong Hwa Kim, Young Ah Kim, Soon Ha Yang |
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Department of Obstetrics and Gynecology, Sungkyunkwan University School of Medicine, Samsung Medical Center, Seoul, Korea. |
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| Abstract |
OBJECTIVE
Nuclear factor kappa B (NF-kappa B) is a transcriptional factor in the expression of cyclooxygenase 2 (COX-2), the key enzyme in production of prostaglandins. The purpose of this study was to investigate the activation of NF-kappa B in human gestational tissues obtained from term pregnant women by various inducers. METHODS: Myometrium, chorion, and amnion were collected during cesarean section from term pregnant women not in labor. Cells from gestational tissues were isolated and cultured with Interleukin-1beta (IL-1beta), Tumor Necrosis Factor-alpha (TNF-alpha) or Lipopolysaccharide (LPS). The activation of NF-kappa B in cells of each tissues was measured by luciferase assay. RESULTS: Luciferase activity analysis showed significantly higher activity of NF-kappa B in myometrial cells treated with LPS, in chorion cells treated with IL-1beta and TNF-alpha, and in amnion cells treated with IL-1beta and LPS than control. CONCLUSION: In cells of gestational tissue at term pregnancy, the activation of NF-kappa B is cell-specific and various according to inducers. |
| Key Words:
NF-kappa B, Myometrium, Amnion, Chorion, Cytokines, Lipopolysacchride |
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