The Expression of p27(kip1) and p57(kip2) in Mouse Endometrium.

Kim, Sung Tae; Lee, Sung Ki; Gye, Myung Chan

Original Article

Korean Journal of Obstetrics & Gynecology 2004;47(3):545-550.
Published online March 1, 2004.

The Expression of p27(kip1) and p57(kip2) in Mouse Endometrium.

Sung Tae Kim, Sung Ki Lee, Myung Chan Gye

¹Department of Obstetrics and Gynecology, Konyang University Hospital, Daejeon, Korea.
²Department of Life Science, College of Natural Sciences, Hanyang University, Seoul, Korea.

Abstract

OBJECTIVE
This study was to investigate the expression of CDK inhibitors, p27(kip1) and p57(kip2) in mouse endometrium during the estrus cycle and pregnant period. METHODS: Total RNA and protein were extracted from endometrium of mouse sacrificed at diestrus, proestrus, estrus, and metestrus cycle, and at day 1-6 post-coitum (p.c.), then semi-quantitative RT-PCR and western blotting of p27(kip1) and p57(kip2) was carried out. RESULTS: p27(kip1) and p57(kip2) mRNA was highly expressed in diestrus and proestrus stage than estrus and metestrus stage. In comparison with estrus cycle, p27(kip1) and p57(kip2) mRNA level was highly maintained in gestational endometrium (except p27(kip1) of day 5 p.c). p57(kip2) protein level was relatively low from day 1 p.c. to day 4 p.c. But it was significantly increased in day 5 p.c. and day 6 p.c. CONCLUSION: These results show that p27(kip1) and p57(kip2) may play a role in endometrial differentiation for regular estrus cycle and implantation, and especially p57(kip2) may play an essential role in endometrial differentiation for maintenance of implantation.

Key Words: Mouse endometrium, p27(kip1), p57(kip2)