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Korean Journal of Obstetrics & Gynecology 2004;47(4):618-626.
Published online April 1, 2004.
Comparison of Protein Expression in Normal Myometrium and Uterine Leiomyoma Using Two-Dimensional Gel Electrophoresis in Korean Women.
Seung Ku Lee, Su Mi Bae, Ko Woon Kim, Min Sook Kim, Eun Kyung Park, Yong Wook Kim, Duck Young Ro, Joon Mo Lee, Seung Eun Namkoong, Chong Kook Kim, Woong Shick Ahn
1Cancer Research Center, Catholic Research Institutes of Medical Science, College of Medicine, The Catholic University of Korea.
2Department of Obstetrics and Gynecology, College of Medicine, The Catholic University of Korea.
3College of Pharmacy, Seoul National University, Korea.
Abstract
OBJECTIVE
Comparison of protein expression by two-dimensional gel electrophoresis (2-DE) in normal myometrium and uterine leiomyoma in Korean women. METHODS: Normal myometrium and uterine leiomyoma tissues were solubilized with 2-DE buffer and the first dimension of PROTEAN IEF CELL, isoelectric focusing (IEF), was performed using pH4-8 linear IPG strips of 17 cm. And then running 12% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS- PAGE) and sliver stain. Scanned image analyzed using PDQuest 2-D softwareTM. Protein spot spectrum was identified by assisted laser desorption/ionization-time of fighting (MALDI-TOF) and the protein mass spectrums identification were performed by searching protein databases of Swiss-prot/TrEMBL, Mascot and MS-FIT. RESULTS: In this study, we found 17 up-regulated proteins (phosphate carrier protein, 60 kDa heat shock protein, acidic calcium-independent, glutathione transferase omega, chloride intracellular channel 4, Ras-related protein Rab-11B, phosphatidylinositol transfer protein alpha isoform, type II keratin subunit protein, Cofilin 2 isoform 1, transgelin, ATP carrier protein, alpha-catenin homolog, parkinson disease 2, apo-cellular retinoic acid binding protein II, osteoglycin preproprotein, proteasome activator subunit 1 isoform, Unnamed protein) and 7 down-regulated proteins (Serum amyloid P component, annexin IV, alpha 1 actin precursor, hypoxanthine-guanine phosphoribosyltransferase, tumor necrosis factor receptor superfamily member EDAR precursor, peroxiredoxin 2, translation elongation factor EF-Tu precursor) between myometrium and leiomyoma. CONCLUSION: 2-DE offer total protein expression between normal myometrium and uterine leiomyoma, and searching of differently expressed protein for the diagnostic markers of leiomyoma.
Key Words: Cancer, Leiomyoma, Myometrium, Two-dimensional gel electrophoresis (2-DE), Matrix assisted laser desorption/ionization-time of flighting (MALDI-TOF)


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