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Korean Journal of Obstetrics & Gynecology 2004;47(5):924-930.
Published online May 1, 2004.
Hydrogen Peroxide-producing Lactobacillus in Vaginal Flora of Pregnant Women with Preterm Labor and Intact Membranes.
Jae Ho Na, Yoon Ha Kim, Jong Suk Oh, Jong Hee Shin, Jung Sun Yang, Cheol Hong Kim, Moon Kyoung Cho, Tae Bok Song
1Department of Obstetrics and Gynecology, Chonnam National University Medical School, Gwangju, Korea.
2Department of Microbiology, Chonnam National University Medical School, Gwangju, Korea.
3Department of Laboratory Medicine, Chonnam National University Medical School, Gwangju, Korea.
Abstract
OBJECTIVE
To investigate the possible role of vaginal infection in preterm delivery, we studied characteristics of vaginal discharge related to hydrogen peroxide producing Lactobacillus. METHODS: Vaginal specimens were obtained from 66 women with normal pregnancy and 30 women with preterm labor and intact membranes. Vaginal pH, leukocyte counts on wet smear, and scores by Nugent criteria on Gram stain were measured. Lactobacillus were tested for production of hydrogen peroxide using a qualitative assay on a tetramethylbenzidine agar plate after incubated for 30 minutes and 1 hour. One hundred eighty-seven hydrogen peroxide-producing Lactobacillus colonies isolated from vaginal fluid of normal pregnant women, and 77 hydrogen peroxide-producing Lactobacillus colonies isolated from it of women with preterm labor and intact membrane. RESULTS: 1. There were no significant differences in vaginal pH between normal pregnant women and women with preterm labor and intact membranes (4.06 +/- 0.31 vs. 4.04 +/- 0.57, p
Key Words: Hydrogen peroxide-producing Lactobacillus, Preterm labor


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