Expression of Angiopoietin-1 and Angiopoietin-2 mRNA in Eutopic and Ectopic Endometrium. |
Ji Young Lee, Seong Soo An, Byung Il Yun, Sung Eun Hur, In Sook Sohn, Soo Nyung Kim |
1Department of Obstetrics and Gynecology, College of Medicine, Konkuk University, Seoul, Korea. 2Department of Obstetrics and Gynecology, College of Medicine, Ewha Woman's University, Seoul, Korea. |
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Abstract |
OBJECTIVE To investigate the mRNA expression of Angiopoietin (Ang)-1 and Ang-2 in ectopic and eutopic endometrium from women with or without endometriosis throughout the menstrual cycle. METHODS: Total RNA was extracted and reverse transcribed into cDNA. The expression of Ang-1 and Ang-2 was examined by quantative competitive PCR (QC PCR) and each result was analyzed by Kruskal- Wallis analysis and Mann-Whitney U-test. RESULTS: The expression of Ang-1 mRNA was statistically significantly higher in endometrium of follicular phase from women with endometriosis compared to that from normal ones in the follicular phase. Endometriotic tissues expressed significantly lower Ang-2 mRNA compared to endometrium from women without endometriosis. CONCLUSION: We suggested that the expression of Ang-1 mRNA could be associated with the pathogenesis of endometriosis and might have a role as neovascularizaton and survival of ectopic endometrium. |
Key Words:
Endometriosis, Angiopoietin-1, Angiopoietin-2 |
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