Factor V Leiden mutation in Korean women with pregnancy-induced hypertension.

Cho, Yong Kyoon; Ko, Ji Kyung; Lee, Chul Min; Choi, Hoon; Kim, Bok Rin; Lee, Hong Kyoon

Review

Korean Journal of Obstetrics & Gynecology 2005;48(4):857-866.
Published online April 1, 2005.

Factor V Leiden mutation in Korean women with pregnancy-induced hypertension.

Yong Kyoon Cho, Ji Kyung Ko, Chul Min Lee, Hoon Choi, Bok Rin Kim, Hong Kyoon Lee

Department of Obstetrics and Gynecology, College of Medicine, Inje University, Sanggye Paik Hospital, Seoul, Korea. ymkcho@sanggyepaik.ac.kr

Abstract

OBJECTIVE
The purposes of this study was to evaluate the frequency of Leiden mutation (missense mutation in the factor V gene at exon 10, 1691 CGA to CAA) in Korean women with well characterized pregnancy-induced hypertension (PIH) compared with normotensive gravid women. METHODS: Genomic DNA from 121 PIH cases and 98 normotensive pregnant control cases were used for polymerase chain reaction (PCR). To genotype Leiden mutation (missense mutation in the factor V gene, exon 10 (1691 G to A)), primers (5'-TGC CCA GTG CTT AAC AAG ACC A-3', 5'-TGT TAT CAC ACT GGT GCT AA-3') were employed to make 267 base pair (bp) PCR product. There was an initial denaturation at 94 degrees C 5 min, followed by 30 cycles of one minute at 94 degrees C, one minute at 55 degrees C, and one minute at 72 degrees C. A 267 bp PCR product was further digested with Mnl I for 2 hour at 37 degrees C and analysed through 12% polyacrylamide gel electrophoresis to determine genotype. Allele 1691G yielded 37 bp, 67 bp, 163 bp fragment and allele 1691A yielded 67 bp, 200 bp fragment. RESULTS: We examined the genotypes of factor V of 121 Korean women with pregnant induced hypertension and 98 normal pregnant women. None of the 219 Korean women carried the factor V Leiden mutation. CONCLUSION: The factor V Leiden mutation is absent and not a common cause of PIH in Korean women.

Key Words: Pregnancy induced hypertension (PIH), Factor V (FV), Leiden mutation, Polymerase chain reaction (PCR), Restriction fragment length polymorphism (RFLP)