| The Protein Oxidation-Stimulating Activities in the Uterine Venous, Umbilical Venous, and Umbilical Arterial Plasma of Preeclampsia. |
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Jae Hoon Kim, Yoon Ha Kim, Cheol Hong Kim, Moon Kyoung Cho, Ki Min Kim, Soo Young Park, Tae Bok Song, Bong Whan Ahn, Sung Yeul Yang |
1Department of Obstetrics and Gynecology, Chonnam National University Medical School, Gwangju, Korea. kimyh@chonnam.ac.kr
2Department of Biochemistry, Chonnam National University Medical School, Gwangju, Korea. |
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| Abstract |
OBJECTIVE
This study was performed to compare the prooxidative activity stimulating the protein carbonyl formation by 3rd generation cephalosporin (moxalactam) and amoxacillin in the uterine venous, umbilical venous, and umbilical arterial plasma of preeclampsia with that of normal pregnancy. METHODS: Lipid peroxide levels in the uterine venous, umbilical venous, and umbilical arterial plasma of normal pregnancy (n=16) and preeclampsia (n=16) were measured by thiobarbituric acid reaction. The basal protein carbonyl contents in the uterine venous, umbilical venous, and umbilical arterial plasma of normal pregnancy (n=16) and preeclampsia (n=16) were determined by the 2,4-dinitrophenylhydrazine (DNPH) method. After plasma of them were mixed and incubated up to 5 hours with 0.2 mL of 1 mM moxalactam or amoxacillin, the protein carbonyl contents in them were measured by DNPH. RESULTS: Lipid peroxide levels in the uterine venous plasma, umbilical venous plasma, and umbilical arterial plasma of preeclampsia were significantly higher than those of normal pregnancy (3.11+/-1.21 vs. 2.18+/-1.16 nmol/mg protein, p<0.05, 5.85+/-1.67 vs. 3.79+/-1.66 nmol/ mg protein, p<0.01, 6.00+/-1.91 vs. 4.99+/-1.78 nmol/mg protein, p<0.01). Protein carbonyls formation by moxalactam in the uterine venous plasma, umbilical venous plasma, and umbilical arterial plasma of preeclampsia were signigicant higher than those of normal pregnancy (19.69+/-8.43 vs. 10.84+/-3.00 nmol/mg protein, p<0.01, 18.94+/-6.96 vs. 10.63+/-1.81 nmol/mg protein, p<0.01, 14.62+/-5.77 vs. 11.21+/-2.08 nmol/mg protein, p<0.05). There were significant positive correlations between lipid peroxide and moxalactam-induced protein carbonyls levels of the uterine venous plasma, umbilical venous plasma, and umbilical arterial plasma (p<0.01). CONCLUSION: These results suggest that increase in the prooxidative activity stimulating the oxidative modification of proteins in utero-placental unit may be involved in the pathogenesis of preeclampsia. |
| Key Words:
Prooxidative activity stimulating the protein carbonyl formation, Lipid peroxide, 3rd generation cephalosporin, Amoxacillin, Preeclampsia |
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