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Korean Journal of Obstetrics & Gynecology 2005;48(8):1942-1949.
Published online August 1, 2005.
Increased Proliferating Activity and Bcl-2 Protein Expression in Eutopic Endometrium with Endometriosis.
Mi Ran Kim, Jae Hoon Lee, Seong Seog Seo, Hye Jin Chang, Kyung Joo Hwang, Young Ah Kim, Dong Wook Park
1Department of Obstetrics and Gynecology, Ajou University School of Medicine, Suwon, Korea. kjhwang@ajou.ac.kr
2Department of Obstetrics and Gynecology, Ilsan Paik Hospital, Inje University, Ilsan, Korea.
3Department of Obstetrics and Gynecology, University of British Columbia, Bancouver, Canada.
Abstract
OBJECTIVE
To evaluate the expression of proliferating activity and Bcl-2 protein property in eutopic endometrium with endometriosis. METHODS: Endometrial tissues were obtained for endometrial biopsy from 2 groups of women. The study groups were 10 samples, which consisted of eutopic endometrial tissue with endometriosis. The remaining 10 samples were control group, which consisted of eutopic endometrial tissue without endometriosis. Expressions of Ki-67 and Bcl-2 protein were immunohistochemically investigated by polyclonal antibody. The computer program (Image pro (+), USA) was used to analysis the staining intensity of tissue samples objectively. Endometrial cells were cultured for 72 hours and the MTT colorimetric assay was used to detect cell proliferation. RESULTS: In the group of eutopic endometrium with endometriosis, the activity of cell proliferation was significantly increased. The expression of Ki-67 protein and MTT assay were significantly higher than those in the control group. And expression of Bcl-2 protein was significantly higher than those in the control group (P<0.05). CONCLUSION: Eutopic endometrium with endometriois showed increasing Bcl-2 protein expression and increasing proliferation. It was believed to be associated with endometrial pathological abnormalities in endometriosis.
Key Words: Endometriosis, Endometrium, Proliferation, Bcl-2 protein


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