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Korean Journal of Obstetrics & Gynecology 2005;48(8):1950-1961.
Published online August 1, 2005.
The relationship between interleukin-1 system gene polymorphism and change in bone mineral density after hormone replacement therapy in postmenopausal Korean women.
Jung Gu Kim, Byung Chul Jee, Seung Yup Ku, Chang Suk Suh, Seok Hyun Kim, Young Min Choi, Shin Yong Moon
Department of Obstetrics and Gynecology, College of Medicine, Seoul National University, Seoul, Korea. kimjg@plaza.snu.ac.kr
Abstract
OBJECTIVE
To investigate the relationship between interleukin-1 (IL-1) system gene polymorphisms, and change in production of IL-1 by whole blood cells (WBCs), and bone mineral density (BMD) after hormone replacement therapy (HRT) in postmenopausal Korean women. METHODS: The IL-1alpha C(-889)T polymorphism, IL-1beta C(-511)T polymorphism and 86-base pair variable number tandem repeat (VNTR) polymorphism in the IL-1 receptor antagonist (IL-1ra) gene was analyzed by restriction fragment length polymorphsim (REFLP), polymerase chain reaction-Genescan, and DNA sequencing in 206 postmenopausal Korean women receiving sequential HRT for 1 year. IL-1alpha, IL-1beta, and IL-1ra produced by WBCs cultured with lipopolysaccharide for 2 days, and serum CrossLaps (CTX), and osteocalcin were measured using enzyme-linked immunosorbent assay (ELISA) and immunoassay respectively. BMD at the lumbar spine and proximal femur was determined by dual energy X-ray absorptiometry. RESULTS: The IL-1 system genotypes were not distributed differently between HRT-responders and HRT-nonresponders (women who lose more than 3% of bone mass per year), and no differences in the annual percent change of BMD after HRT were noted among IL-1 system genotypes. After HRT, the production of IL-1alpha by WBCs decreased but no changes in the production of other components of IL-1 system were noted. There were no differences in the 6 month changes of bone turnover markers and the production of IL-1 system components by WBCs and IL-1beta/IL-1ra ratio after HRT across IL-1 system genotypes. Except a negative correlation between IL-1alpha with annual change of BMD at trochanter after HRT, the production of other components of IL-1 system by WBCs and their changes after HRT of 6 month did not correlated with annual change of BMD after HRT CONCLUSION: The IL-1 system gene polymorphisms do not affect change in BMD, and change in the production of ILs by WBCs after HRT in postmenopausal Korean women.
Key Words: Postmenopausal women, IL-1 system, Gene polymorphism, Bone mineral density, Hormone therapy


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