Korean J Obstet Gynecol Search

CLOSE


Korean Journal of Obstetrics & Gynecology 2005;48(12):2910-2918.
Published online December 1, 2005.
Correlation of HLA-G Gene Expression with Various Prognostic Factors in the Tissue of Ovarian Carcinoma.
Won Ki Hong, Young Tae Kim, Bo Sung Yoon, Sang Wun Kim, Sung Hoon Kim, Jae Hoon Kim, Jae Wook Kim, Yong Won Park
Department of Obstetrics and Gynecology, Institute of Women's Health Science, Women's Cancer Clinic, Yonsei University College of Medicine, Seoul, Korea. ytkimchoi@yumc.yonsei.ac.kr
Abstract
OBJECTIVE
The objective of this study was to investigate the relationship between the expression of mRNA and protein of HLA-G and other clinicopathologic prognostic factors of ovarian cancer. METHODS: 43 patients diagnosed with ovarian cancer from 1997 to 2005 and 5 patients with normal ovarian tissue (controls) were enrolled in this prospective study. The patient group all went through baseline studies with staging laparotomy and adjuvant chemotherapy. Quantitative real-time RT PCR and Western blot analysis were used in detecting the expression of mRNA and protein of HLA-G. RESULTS: The mRNA expression of HLA-G (2-delta delta Ct value) in cancer group were 1.21 (0-9), and 0.01 (0-0.02) in control group, which was statistically significant (P=0.005). The expressed protein levels did not show any difference between the cancer and control groups. There also was a significant relationship between the serum CA 125 at the time of diagnosis and the HLA-G protein levels (P=0.02). But the relationship between other clinicopathologic prognostic factors and the HLA-G protein levels were not statistically significant. CONCLUSION: Our results showed that HLA-G mRNA expression level was much higher in ovarian cancer patient group than in those of control group. Therefore HLA-G maybe play an important role in carcinogenesis of ovarian cancer. Although the protein level of HLA-G had low significance with other prognostic factors, serum CA 125 showed a statistically significant relation with protein levels of HLA-G. Further studies based on the correlation between HLA-G and survival rate are needed to support HLA-G as a prognostic factor of ovarian cancer.
Key Words: Ovarian cancer, HLA-G gene, CA-125, Prognostic factors


ABOUT
ARTICLE & TOPICS
Article category

Browse all articles >

Topics

Browse all articles >

BROWSE ARTICLES
POLICY
FOR CONTRIBUTORS
Editorial Office
4th Floor, 36 Gangnam-daero 132-gil, Gangnam-gu, Seoul 06044, Korea.
Tel: +82-2-2266-7238    Fax: +82-2-3445-2440    E-mail: journal@ogscience.org                

Copyright © 2024 by Korean Society of Obstetrics and Gynecology.

Developed in M2PI

Close layer
prev next