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Korean Journal of Obstetrics & Gynecology 2006;49(7):1492-1500.
Published online July 1, 2006.
Comparison of two culture media on in vitro maturation and fertilization of immature oocytes obtained from stimulated cycles.
Sang Hoon Han, Hyun Jun Kim, Jung Hee Moon, Byung Chul Jee, Seung Yup Ku, Chang Suk Suh, Seok Hyun Kim, Yong Min Choi, Jung Ku Kim, Shin Yong Moon
1Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital, Seongnam, Korea. blasto@snubh.org
2Department of Obstetrics and Gynecology, College of Medicine, Seoul National University, Seoul, Korea.
3Institute of Reproductive Medicine and Population, Medical Research Center, Seoul National University, Seoul, Korea.
Abstract
OBJECTIVE
The optimal culture conditions for human immature oocytes are still investigating. We compared retrospectively the efficacy of two culture media (pyruvate-dominant YS media vs glucose-dominant G2 media) on in vitro maturation and fertilization rate of immature oocytes obtained from stimulated IVF cycles. METHODS: One hundred forty-three immature oocytes (including GV and metaphase I) were obtained from 67 cycles of IVF-ET (52 patients). The mean age of female was 32.5+/-3.7 years. Ovarian hyperstimulation was performed using hMG or rFSH with GnRH antagonist and then ovulation was triggered by urinary or rhCG. Immature oocytes were cultured for 4-24 hrs and then fertilized by ICSI. We used YS media between July 2003 and September 2004, and G2 media between October 2004 and August 2005. Each media was prepared containing 30% of patients' follicular fluid, rFSH (1 IU/mL), rLH (1 IU/mL) and rEGF (10 ng/mL). RESULTS: The in vitro maturation rate of immature oocytes (67% vs 66%) and their fertilization rate (61% vs 61%) was not different between YS and G2 media. CONCLUSION: Immature oocytes seem to have a similar developmental competency in each of pyruvate dominant YS and glucose dominant G2 media containing human follicular fluid. Further investigations will be warranted to find the optimal media which can increase the developmental potential of immature oocytes.
Key Words: Immature oocyte, In vitro maturation, Controlled ovarian hyperstimulation, Fertilization


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