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Korean Journal of Obstetrics & Gynecology 2007;50(1):101-110.
Published online January 1, 2007.
Establishment and characterization of secondary drug resistant cell line originating from cisplatin-resistant SNU-8.
Chun San An, Sung Yob Kim
Department of Obstetrics and Gynecology, College of Medicine, Cheju National University, Cheju, Korea. kimsy@cheju.ac.kr
Abstract
OBJECTIVE
Multidrug resistance to chemotherapy is a major obstacle in attempts to improve the clinical outcome of ovarian carcinoma patients. The aim of this study is to establish secondary anticancer drug resistant cell line from original SNU-8/WT resistant to cisplatin and characterize it. METHODS: After establishing secondary drug resistant cell line (SNU-8/Fac), drug sensitivity was measured by MTT assay. Multidrug resistance (MDR) was analyzed by RT-PCR and western blotting analysis. RESULTS: MTT assay data demonstrated that MDR was expressed in SNU-8/Fac. In addition, mRNA expression of MDR1, ATP7B in SNU-8/Fac was increased. However, overexpression of MRP1, BCRP, TS and MT mRNA was not observed. At the protein level, protein P-gp, ATP7B were overexpressed in SNU-8/Fac. CONCLUSION: We established a new anticancer drug resistant cell line from original SNU-8/WT. Overexpression of P-gp and ATP7B was observed in SNU-8/Fac.
Key Words: Ovarian cancer, Multidrug resistance, P-gp/MDR1, ATP7B, Cisplatin


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