| The expression and interaction of HLA-G and IL-10 in cervical cancer. |
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Young Tae Kim, Bo Sung Yoon, Jae Wook Kim, Sung Hoon Kim, Jae Hoon Kim, Sang Wun Kim, Eun Ji Nam |
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Department of Obstetrics and Gynecology, Women's Cancer Clinic, Yonsei University College of Medicine, Seoul, Korea. ytkchoi@yumc.yonsei.ac.kr |
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| Abstract |
OBJECTIVES
To determine the expression of HLA-G and IL-10 and their correlation in tissue of cervical cancer, and to investigate the relationship between their expression and clinicopathologic factors in patients with cervical cancer. METHODS: Tissue samples were obtained from 40 patients diagnosed with cervical cancer and 15 patients with normal cervix for control from Oct. 2004 to Oct. 2005. Quantitative real-time RT-PCR for HLA-G mRNA and semi-quantitative RT-PCR for IL-10 mRNA were used. And proteins of HLA-G and IL-10 were detected by Western blot analysis. RESULTS: Both HLA-G and IL-10 mRNA expression in cervical cancer tissue were higher than normal control, which was statistically significant (P<0.001, P<0.001). The proteins levels of HLA-G and IL-10 in cancer group were also significantly higher than control (P<0.001, P=0.041). The mRNA expression of HLA-G tended to be correlated with IL-10 expression (P=0.061), although it was not statistically significant. Among clinicopathologic factors of cervical cancer, there was inverse relationship between FIGO stage and mRNA value of HLA-G (P=0.045). CONCLUSIONS: The mRNA and protein expression of HLA-G and IL-10 in cervical cancer were much higher than in controls. These results showed that HLA-G and IL-10 might have an important role of tumorigenesis in patients with cervical cancer. The levels of HLA-G and IL-10 seem to be correlated although it was not statistically significant. High HLA-G mRNA expression could be related in early tumorigenesis since it was associated with early stage cervical cancer. |
| Key Words:
human leukocyte antigen-G, interleukin-10, cervical cancer |
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