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Korean Journal of Obstetrics & Gynecology 2007;50(3):429-438.
Published online March 1, 2007.
Association of the vascular endothelial damage and estrogen, progesterone.
Mi Kyoung Kim, Ji Ae Kim, Yeo Jin Jeon, Jong Soon Park, Mi Hye Park, Suk Hyo Suh, Sun Hee Chun, Jung Ja Ahn, Young Ju Kim
1Department of Obstetrics and Gynecology, College of Medicine, Ewha Womans' University, Seoul, Korea. kkyj@ewha.ac.kr
2Department of Physiology, College of Medicine, Ewha Womans' University, Seoul, Korea.
3Ewha Medical Research Center, College of Medicine, Ewha Womans' University, Seoul, Korea.
4Ewha Global Challenge for Medicine Ewha Womans' University, Seoul, Korea.
Abstract
OBJECTIVE
The aim of this study were to examine the serum level of estradiol, estriol, progesterone, oxidized LDL in preeclamtic patients and to evaluate the protective effects of estrogen and progesterone against lysophosphatidylcholine (LPC) induced cell death in Human umbilical vein endothelial cells (HUVECs). METHODS: We analysed the serum level of estradiol, estriol, progesterone, oxidized LDL in patients with preeclampsia and control. We used LPC to induce cell death in HUVECs. For cytotoxic assay, we did LDL assay for cell death and Resazurin assay for cell viability. HUVECs were exposed to various concentrations of LPC, LPC+estrogen, LPC+progesterone and we did cytotoxic assay. RESULTS: The serum estradiol, estriol were lower in the preeclamptic patients (P<0.05). Oxidized LDL were higher in the preeclamptic patients(P<0.05). LPC induced cell death in a concentration-dependant manner. Estrogen or progesterone inhibited LPC-induced cell death in a concentration-dependant manner (P<0.05). CONCLUSION: Estrogen and progesterone attenuated LPC-induced cytotoxicity. The results suggest that Oxidized LDL induced endothelial damage in preeclampsia may be induced by low serum estradiol, estriol and progesterone levels and prevented by estrogen and progesterone addition.
Key Words: Endothelial damage, Estrogen, Progesterone


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