| Increased protein carbonyls as markers for oxidative stress in amniotic fluid of preeclamptic women at term. |
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Byoung Jae Kim, Joong Shin Park |
1Department of Obstetrics and Gynecology, Seoul National University College of Medicine, Korea. jsparkmd@snu.ac.kr
2Department of Obstetrics and Gynecology, Seoul National University Boramae Hospital, Seoul, Korea. |
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| Abstract |
OBJECTIVE
To determine if the content of protein carbonyls in amniotic fluid is elevated in preeclamptic women at term. METHODS: A case-control study was conducted to compare contents of protein carbonyls in term amniotic fluid (gestational age at amniocentesis > or = 37 wks) among women who were healthy pregnant, preeclamptic and chronic hypertensive. Protein carbonyls were measured by specific immunoassay. Nonparametric techniques were used for statistical analysis. RESULTS: 1) The median amniotic fluid protein carbonyl of preeclamptic women was significantly higher than that of normal pregnant women (median 0.663 nmol/mg, range 0.013-1.726 nmol/mg vs. median 0.395 nmol/mg, range 0.069-1.503 nmol/mg, p<.05). 2) There was no significant difference in the amniotic fluid protein carbonyl level between women with chronic hypertension and normal pregnant women (median 0.823 nmol/mg, range 0.064-1.575 nmol/mg vs. median 0.395 nmol/mg, range 0.069-1.503 nmol/mg, p=.14). 3) The median amniotic fluid protein carbonyl showed tendency to increase with severity of preeclampsia without reaching statistical significance (normal pregnancy, median 0.395 nmol/mg, range 0.069-1.503 nmol/mg; mild preeclampsia, median 0.663 nmol/mg, range 0.013-1.054 nmol/mg; severe preeclampsia, median 0.842 nmol/mg, 0.391-1.726 nmol/mg; p=.07). The median protein carbonyl in amniotic fluid of women with severe preeclampsia was significantly higher than that of normal pregnant women (median 0.842 nmol/mg, range 0.391-1.726 nmol/mg vs. median 0.395 nmol/mg, range 0.069-1.503 nmol/mg, p<.05). CONCLUSIONS: The protein carbonyls increased in amniotic fluid of women with preeclampsia, but not chronic hypertension at term. |
| Key Words:
Preeclampsia, Amniotic fluid, Protein carbonyl formation, Oxidative stress, Hypertension |
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