Korean J Obstet Gynecol Search

CLOSE


Korean Journal of Obstetrics & Gynecology 2007;50(11):1493-1498.
Published online November 1, 2007.
Inhibition of clusterin gene expression via shRNA increases chemosensitivity to paclitaxel in xenografted PEOH cells.
Eun Young Shin, In Cheal Jeung, Joo Hyuk Choi, Dong Choon Park
1Research Institute of Medical Science of Saint Vincent Hospital, The Catholic University of Korea, Korea. dcpark@catholic.ac.kr
2Department of Obstetrics and Gynecology, St. Vincent's and St. Mary's Hospital, The Catholic University of Korea.
Abstract
OBJECTIVE
To evaluate the inhibition of Clusterin gene expression via shRNA decreases proliferation and metastasis and increases chemosensitivity to paclitaxel in xenografted PEOH cells. METHODS: 1 x 10(6) paclitaxel resistant cell lines transduced with Clusterin shRNA in lentiviral inoculated subcutaneously into the flank region of 6 to 8 week-old female nude mice. Parental cells transduced with LacZ was used as a control. Tumor growth was measured twice every week and calculated by using the formula: length x width x depth x 0.5236. The mice were sacrificed and examined for Clusterin expression on tumor cells and counted the metastasis sites. RESULTS: shRNA for Cluaterin works in vivo and it is the in accord with the in vitro results. Although shRNA for Clusterin group showed decreased tumor growth and proliferation it has not statistical significance. But transfection of Clusterin shRNA on PEOH significantly increased paclitaxel-sensitivity (P<0.05). CONCLUSION: shRNA targeting of the Clusterin gene decreased the ovarian cancer cell's paclitaxel resistance.
Key Words: Clusterin, Ovarian cancer, shRNA, Paclitaxel resistance
TOOLS
Share :
Facebook Twitter Linked In Google+ Line it
METRICS Graph View
  • 143 View
  • 0 Download
Related articles in Obstet Gynecol Sci


ABOUT
ARTICLE & TOPICS
Article category

Browse all articles >

Topics

Browse all articles >

BROWSE ARTICLES
POLICY
FOR CONTRIBUTORS
Editorial Office
4th Floor, 36 Gangnam-daero 132-gil, Gangnam-gu, Seoul 06044, Korea
Tel: +82-2-3445-2382    Fax: +82-2-3445-2440    E-mail: journal@ogscience.org                

Copyright © 2021 by Korean Society of Obstetrics and Gynecology. All rights reserved.

Developed in M2PI

Close layer
prev next