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Korean Journal of Obstetrics & Gynecology 2007;50(11):1493-1498.
Published online November 1, 2007.
Inhibition of clusterin gene expression via shRNA increases chemosensitivity to paclitaxel in xenografted PEOH cells.
Eun Young Shin, In Cheal Jeung, Joo Hyuk Choi, Dong Choon Park
1Research Institute of Medical Science of Saint Vincent Hospital, The Catholic University of Korea, Korea. dcpark@catholic.ac.kr
2Department of Obstetrics and Gynecology, St. Vincent's and St. Mary's Hospital, The Catholic University of Korea.
To evaluate the inhibition of Clusterin gene expression via shRNA decreases proliferation and metastasis and increases chemosensitivity to paclitaxel in xenografted PEOH cells. METHODS: 1 x 10(6) paclitaxel resistant cell lines transduced with Clusterin shRNA in lentiviral inoculated subcutaneously into the flank region of 6 to 8 week-old female nude mice. Parental cells transduced with LacZ was used as a control. Tumor growth was measured twice every week and calculated by using the formula: length x width x depth x 0.5236. The mice were sacrificed and examined for Clusterin expression on tumor cells and counted the metastasis sites. RESULTS: shRNA for Cluaterin works in vivo and it is the in accord with the in vitro results. Although shRNA for Clusterin group showed decreased tumor growth and proliferation it has not statistical significance. But transfection of Clusterin shRNA on PEOH significantly increased paclitaxel-sensitivity (P<0.05). CONCLUSION: shRNA targeting of the Clusterin gene decreased the ovarian cancer cell's paclitaxel resistance.
Key Words: Clusterin, Ovarian cancer, shRNA, Paclitaxel resistance
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