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Korean Journal of Obstetrics & Gynecology 2009;52(3):314-321.
Published online March 1, 2009.
The effects of bisphosphonates on differentiation of human amniotic fluid-derived mesenchymal stem cells into osteoblast.
Joon Hyop An, Byeong Min An, Tae Young Choi, Sang Gook Kim, Hwa Sook Moon, Bo Sun Joo
1Division of Endocrinology and Metabolism, Department of Internal Medicine, Good Moonhwa Hospital, Busan, Korea.
2Center for Reproductive Medicine, Good Moonhwa Hospital, Busan, Korea. bosunjoo@hanmail.net
3Department of Obstetrics and Gynecology, Good Moonhwa Hospital, Busan, Korea.
It has been known that amniotic fluid (AF) is rich source of mesenchymal stem cells (MSCs). Bisphosphonates are widely used in clinical treatment of various metabolic bone diseases and their primary action is the inhibition of osteoclastic bone resorption. However, litter is known about whether bisphosphonates affect the differentiation into osteoblast, especially from AF-derived MSCs (AFMSCs). Therefore, the purpose of this study is to investigate whether these bisphosphonates influence in the process of AFMSCs differentiation into osteoblast. METHODS: AF samples were obtained by second trimester amniocentesis for fetal karyotyping from 6 pregnant women. Cells were treated with various concentration (0, 10(-10), 10(-8), 10(-6) M) of zoledronate and alendronate and analyzed over 21 days of culture. Differentiation into osteoblast was determined by cell staining and RT-PCR for alkaline phosphatase (ALP). RESULTS: It was observed that AFMSCs could differentiate into osteoblast. Alendronate had more potent effect than zoledronate in osteoblastic differentiation. ALP expression was increased with increasing concentration of zoledronate and it was highest in 10(-8) M alendronate. However, no effect of bisphosphonates was found in 14 days of culture. CONCLUSION: This study shows that AFMSCs can be differentiated into osteoblast. The induction of these differentiation following bisphosphonate treatment was appear to be drug type-, dose-, and culture time-dependent. However, further studies are needed to conclude a consistent outcome for the effects of bisphosphonate on differentiation potential of AFMSCs.
Key Words: Amniotic fluid, Mesenchymal stem cell, Zoledronate, Alendronate, Osteoblast

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