Ovarian cancer related gene targeting with large circular antisense library. |
Kyung Oh Doh, Geun Soo Chun, Jae Chul Sim, Hoe Saeng Yang |
1Department of Obstetrics and Gynecology, College of Medicine Dongguk University, Gyeongju, Korea. y31354@hotmail.com 2Department of Physiology, Yeungnam University College of Medicine, Daegu, Korea. |
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Abstract |
OBJECTIVE The aim of this study is to find out the genes which are related to ovarian cancer cell growth using large circular antisense library. METHODS: Clones for antisense library were uni-directionally sub-cloned into pBS SK (-) vector. LC-antisense molecules were then purified from the culture supernatants of the bacterial competent cells superinfected with M13K07 helper bacteriophages. The LC-antisense library to 240 unigene clone was constructed and utilized in the identification of genes functionally involved in the growth of ovarian cancer cells. RESULTS: The 17 numbers out of the 240 numbers of the antisense library exerted a marked inhibitory effect on the growth of SK-OV 3. CONCLUSION: The putative functional categorization of each gene was then conducted via public databases. These candidates may be used as target genes for drug development or adjuvant of conventional chemotherapeutic drugs. |
Key Words:
Ovarian neoplasms, Large circular antisense DNA, Gene therapy |
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