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Obstet Gynecol Sci > Volume 53(1); 2010 > Article
Korean Journal of Obstetrics & Gynecology 2010;53(1):35-42.
DOI: https://doi.org/10.5468/kjog.2010.53.1.35    Published online January 1, 2010.
Comparison of cell growth suppression in SiHa cervical carcinoma cell line by human papillomavirus type 16 E6/E7 siRNAs.
Sae Hyun Park, Byung Joon Park, Yong Wook Kim, Duck Yeong Ro, Tae Eung Kim, Jae Keun Jung, Su Mi Bae, Woong Shick Ahn
1Department of Obstetrics and Gynecology, College of Medicine, The Catholic University of Korea, Seoul, Korea. ahnlab1@catholic.ac.kr
2Catholic Research Institutes of Medical Science, The Catholic University of Korea, Seoul, Korea.
Abstract
OBJECTIVE
Human cervical cancer is caused by the high-risk types of human papillomavirus (HPV) such as HPV16, which possess the E6 and E7 oncogenes, whose expressions are a prerequisite for cancer development. We performed this study to compare the efficacy of antitumor activity by HPV siRNA which silences only E6 or both E6/E7. METHODS: We transfected siRNA 377 (HPV16 E6 siRNA), siRNA 3 (HPV16 E6 siRNA), and siRNA 198 (HPV16 E7 siRNA) into SiHa cell line (siRNA 377 silences only E6, and siRNA 3 and siRNA 198 silence both E6 and E7). We experimented cell counts and morphologic changes 24 and 48 hours after transfection and expressions of HPV16 E6/E7 mRNA by RT-PCR. RESULTS: siRNA 377, siRNA 3, and siRNA 198 suppressed the cell growth. siRNA 3 and siRNA 198 were more potent than siRNA 377 in cell growth suppression. siRNA 377 knocked down the expression of E6 mRNA, and both siRNA 3 and siRNA 198 knocked down the expression of E6/E7 mRNA. CONCLUSION: Our results suggest that simultaneous suppression of E6 and E7 was more potent than E6-specific suppression in cancer cell growth.
Key Words: Cervical cancer, Human papillomavirus, E6, E7, siRNA


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