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Obstet Gynecol Sci > Volume 53(10); 2010 > Article
Korean Journal of Obstetrics & Gynecology 2010;53(10):894-904.
DOI: https://doi.org/10.5468/kjog.2010.53.10.894    Published online October 1, 2010.
Detection of human papillomavirus type 16 and 18 by PCR in patients with cervical neoplasia.
Choong Hak Park, Jae Kyoung Kim
1Department of Obstetrics and Gynecology, Dankook University College of Medicine, Cheonan, Korea. pch10@dankook.ac.kr
2Department of Medical Science of Diagnostic Test, Dankook University Hospital, Dankook University College of Medicine, Cheonan, Korea.
The purpose of this study was to evaluate the detection rate of human papillomavirus (HPV) DNA Test (type 16 and 18) and to investigate the clinical significance of HPV DNA test in patients with cervical neoplasia. METHODS: Of the 708 patients aged 21~83 years who had undergone both conventional Papanicolaou cervical cytologic test and HPV DNA test by polymerase chain reaction, 383 cases underwent histologic diagnoses. RESULTS: Of the 708 cytologic diagnoses, there were 11 positive HPV DNA test diagnoses in squamous cell carcinoma (SCC), 41 in high-grade squamous intraepithelial lesion (HSIL), 20 in low-grade squamous intraepithelial lesion (LSIL), 41 in atypical squamous cells (ASC), and 86 in negative cytology. Of the 383 histologic diagnoses, there were 24 positive HPV DNA test diagnoses in SCC, 42 in cervical intraepithelial neoplasia (CIN) 3, 12 in CIN 2, 12 in CIN 1, 7 in atypical change, and 45 in negative histology. Of the 239 patients with negative HPV DNA test, 28.5% cases showed histologic diagnoses of CIN 1 or worse lesion. Of the 46 patients with negative cytology and positive HPV DNA test, 50.0% cases showed histologic dianoses of CIN 1 or worse lesion. Pap cytology revealed sensitivity of 72.5%, specificity of 66.4%, and false negative rate of 27.5%, whereas HPV DNA test showed 57.5%, 76.7%, and 42.5%, respectively. CONCLUSION: This study confirmed that the primary standard Pap cytology and HPV DNA test were adjunctive. Also this study showed that physicians always should not overrate Pap cytology or HPV DNA test in managing the patient with cervical neoplasia. Combined test was a very effective diagnostic method for detecting cervical neoplasia.
Key Words: Cervical neoplasia, HPV DNA test, Pap cytology, PCR

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