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Obstet Gynecol Sci > Volume 53(10); 2010 > Article
Korean Journal of Obstetrics & Gynecology 2010;53(10):905-914.
DOI: https://doi.org/10.5468/kjog.2010.53.10.905    Published online October 1, 2010.
The effect of granulocyte-macrophage-stimulating factor on the gene expression in human trophoblast of early pregnancy.
Hun Yul Lee, Sang Soo Kim, Chang Suk Ahn, Young Joo Park, Jin Hyun Jun, Haengsuk Song, Hye Sung Moon, Eun Joo Park, Seo You Hong, Won Il Park
1Department of Obstetrics and Gynecology, Eulji University School of Medicine, Daejeon, Korea. pwi3110@eulji.ac.kr
2Life Science Institute, Eulji University School of Medicine, Daejeon, Korea.
3Laboratory of Molecular Oncology, Cheil General Hospital, Kwandong University College of Medicine, Seoul, Korea.
4Department of Obstetrics and Gynecology, Ewha Woman's University School of Medicine, Seoul, Korea.
Abstract
OBJECTIVE
Granulocyte-macrophage colony-stimulating factor (GM-CSF) facilitates mammalian embryonic development and implantation. However, its biological function after implantation is not elucidated. The aim of this study is to assess the changes of gene expression by GM-CSF in human trophoblast obtained in early pregnancy. METHODS: Human trophoblast obtained in early pregnancy was cultured with or without GM-CSF. The difference of gene expression was evaluated with microarray and selected genes were reevaluated with real-time reverse transcription-polymerase chain reaction (RT-PCR). RESULTS: Microarray analysis revealed that the expressions of 468 genes were increased while those of 40 genes were decreased by GM-CSF. These genes were evaluated according to the known biologic pathways. The regulation of actin cytoskeleton and focal adhesion pathways were mostly influenced by GM-CSF. Annexin A2, thymosin-like 3, vimentin, myogenin, ACK1, and tensin1 genes were selected for real-time RT-PCR. The increased expressions of of vimentin and ACK1, and decreased expressions of tensin1 were confirmed by real-time RT-PCR. CONCLUSION: GM-CSF activates focal adhesion pathway in human trophoblast by increasing the expression of vimentin and ACK1, and decreasing the expression of tensin1.
Key Words: Granulocyte-macrophage colony-stimulating factor, Trophoblast, Focal adhesion, Invasion


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