Gene expression of heme oxygenase-1 and nitric oxide synthase on trophoblast of preeclampsia.

Choi, Song Kwon; Hwang, Jong Yun; Lee, Jiyeon; Na, Sung Hun; Ha, Joong Gyu; Lee, Hyang Ah; Lee, Dong Hun

doi:2011.54.7.341

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Obstet Gynecol Sci > Volume 54(7); 2011 > Article

Original Article

Korean Journal of Obstetrics & Gynecology 2011;54(7):341-348.
DOI: https://doi.org/10.5468/KJOG.2011.54.7.341 Published online July 1, 2011.

Gene expression of heme oxygenase-1 and nitric oxide synthase on trophoblast of preeclampsia.

Song Kwon Choi, Jong Yun Hwang, Jiyeon Lee, Sung Hun Na, Joong Gyu Ha, Hyang Ah Lee, Dong Hun Lee

Department of Obstetrics and Gynecology, Kangwon National University School of Medicine, Chuncheon, Korea. rapidhwang@kangwon.ac.kr

Abstract

OBJECTIVE
The aim of this study was to evaluate the gene expression of heme oxygenase-1 (HO-1), inducible nitric oxide synthase (iNOS) and endothelial nitric oxide synthase (eNOS) in placenta of preeclampsia. METHODS: Placenta were obtained from women with normal pregnancies (n=15) and severe preeclamptic (n=15) after informed consent and under the approval of IRB of Kangwon National University Hospital. The severe preeclampsia was diagnosed as high blood pressure and proteinuria. High blood pressure was defined as systolic blood pressure of >160 mm Hg or diastolic pressure of >110 mm Hg and proteinuria was defined as urine protein of >2 g/24 hr. We investigated mRNA expression of HO-1, iNOS and eNOS in both groups by real time polymerase chain reaction and immunohistochemistry. The Student's t-test was used for statistical analysis with SPSS ver. 12.0. P<0.05 was considered to be statistically significant. RESULTS: There were no significant differences in maternal age (P=1.181) and gestational age (P=0.30) between the two groups. Fetal birth weight from women with preeclampsia was significantly lower than that from normal pregnant women (P=0.002). We detected differences in mRNA expression of placenta between both groups. Expression of HO-1 was decreased in placenta of preelamptic pregnancies compared to that from normal. However, expression of iNOS and eNOS was higher in preeclamptic group compared to normal. CONCLUSION: We suggest that preeclampsia is associated with a decreased expression of HO-1 and increased expressions of iNOS and eNOS in placenta.

Key Words: Preeclampsia, Cytotrophoblast, Heme oxygenase-1

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