| Effect of EGF and TGF- on Cellular Adhesion and the Expression of E-cadherin and EGF Receptor in Cervical Cancer Cell Lines. |
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J H Shin, H S Moon, H Y Park, J I Kim, H K Yoo |
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| Abstract |
OBJECTIVE
Invasive squamous cell carcinoma is known to have undifferentiated epithelial cell, decreased cell adhesion and increased invasiveness of tumor cell and decreased expression of cell adhesive factors known as E-cadherin. And also, the over-expression of growth factor receptors, such as EGF receptor [EGFR], was observed in invasive lung cancer and esophageal cancer. The purpose of this study was to determine the effect of E-cadherin, growth factors, and their receptor in the invasion and metastasizing process of cervical cancer. METHODS: Among the cervical cancer cell lines, CaSki, SiHa, ME-180, HeLa, HT-3 and C-33A cell lines were cultured and observed for morphological change of tumor cell. A western blot analysis was performed for detecting E-cadherin, EGFR and activated EGFR. The cervical cancer cell cultures were treated with different time duration [0, 10 min, 20 min, 30 min, 1 hr, 2 hr, 4 hr, 8 hr, 24 hr] and the change in E-cadherin, EGFR and activated EGFR expression were studied. RESULTS:CaSki, HT-3, and ME-180 cell lines showed epithelial contact growth, while SiHa, HeLa and C-33A cell lines showed fibroblastoid growth. Through western blot analysis, E-cadherin 120 kDa band and EGFR 170 kDa band were expressed in CaSki, HT-3 and ME-180 cell line, which showed epithelial contact growth. In these 3 cell lines, expression of E-cadherin did not decrease with time dependent manner. Expression of EGFR decreased and activated EGFR expression increased in 30 minutes to 1 hour but decreased subsequently. CONCLUSION: This study showed that the change in the activity of E-cadherin is more important in the invasion and metastasis of cervical cancer rather than the decrease of E-cadherin. The change of EGFR resulting from stimulation of growth factors such as EGF, TGF- , especially the function of activated EGFR associated in tyrosine phosphorylation is important in the signal transduction of inducing invasion of cervical cancer cell. |
| Key Words:
Invasion, Cervical cancer, E-cadherin, EGFR |
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