Prenatal Diagnosis of Duchenne Muscular Dystrophy (DMD) by Multiplex Polymerase Chain Reaction (PCR) with Chorionic Villi.

Lee, Kyo Won; Yoon, Seon Woong; Chang, Woo Sup; Kim, Jin Yeong; Lee, Sook Hwan; Lee, Sang Joon; Kim, Sung Do; Han, Jong Sul

Original Article

Korean Journal of Obstetrics & Gynecology 1999;42(9):2028-2032.
Published online January 1, 2001.

Prenatal Diagnosis of Duchenne Muscular Dystrophy (DMD) by Multiplex Polymerase Chain Reaction (PCR) with Chorionic Villi.

Kyo Won Lee, Seon Woong Yoon, Woo Sup Chang, Jin Yeong Kim, Sook Hwan Lee, Sang Joon Lee, Sung Do Kim, Jong Sul Han

Abstract

OBJECTIVE
To evaluate the efficacy of prenatal multiplex PCR with chorionic villi, in the case of family history of DMD due to exon deletion. METHODS: DNA was extracted when cells' size are 1x 106 on T- flask surface area reach to 25 cm2 after 2 weeks from sex confirmation. Average DNA concentration was 50-100 ng and multiplex PCR test was performed from DNA extraction. RESULT: PCR was done for 17 exons devided into 4 groups. Seventeen exons were all amplified with their right size. CONCLUSIONS: This method is DNA analysis for prenatal diagnosis of DMD with chorionic villi in the family of DMD. This is useful when preimplantation genetic diagnosis is not available.