Cumulative Pregnancy Rate of In Vitro Fertilization and Embryo Transfer With Intracytoplasmic Sperm Injection.

Kim, Suk Hyun; Shim, Soon Sub; Jee, Byung Chul; Choi, Sung Mi; Kim, Hee Sun; Ryu, Bum Yong; Oh, Sun Gyung; Seo, Chang Suk; Choi, Young Min; Bae, Gwang Bum; Kim, Jung Goo; Moon, Sin Yong; Lee, Jin Yong

Original Article

Korean Journal of Obstetrics & Gynecology 2001;44(1):48-56.
Published online January 1, 2001.

Cumulative Pregnancy Rate of In Vitro Fertilization and Embryo Transfer With Intracytoplasmic Sperm Injection.

Suk Hyun Kim, Soon Sub Shim, Byung Chul Jee, Sung Mi Choi, Hee Sun Kim, Bum Yong Ryu, Sun Gyung Oh, Chang Suk Seo, Young Min Choi, Gwang Bum Bae, Jung Goo Kim, Sin Yong Moon, Jin Yong Lee

¹Department of Obstetrics and Gynecology, College of Medicine, Seoul National University, Seoul, Korea.
²Institute of Reproductive Medicine and Pop ulation , Medical Research Center, Seoul National University, Seoul, Korea.

Abstract

OBJECTIVE
To compare the clinical outcomes between Day 2 and Day 3 embryo transfer(ET) groups in in vitro fertilization and embryo transfer(IVF-ET) with intracytoplasmic sperm injection(ICSI). METHODS: From May, 1997 to December, 1998, 174 cycles of IVF-ET with ICSI were performed and classified into two groups: Day 2 ET group(n=134) and Day 3 ET group (n=40). In Day 3 ET group, embryos fertilized after ICSI were cultured in vitro for further 24 hours in M3 media. RESULTS: There were no significant differences in the age and BMI of patients, basal serum FSH level, protocol of controlled ovarian hyperstimulation(COH), indication of ICSI, and source of sperm for ICSI between two groups. Only the number of the previous failed IVF-ET cycles was significantly higher in Day 3 ET group(p<0.05). Serum E2 level on hCG day, the numbers of oocytes retrieved after COH, oocytes fertilized after ICSI, and embryos transferred, and the rates of fertilization, cleavage, and implantation showed no significant differences. However, cumulative embryo score(CES) was significantly higher in Day 3 ET group(p<0.05). Although there were no significant differences in the rates of pregnancy per ET, spontaneous abortion, and live birth, the rates of biochemical and multiple pregnancy were significantly higher in Day 3 ET group(p<0.05). CONCLUSIONS: In IVF-ET with ICSI, the relatively higher CES may contribute to the higher risk of multiple pregnancy in Day 3 ET group, compared with the conventional Day 2 ET group.

Key Words: In vitro fertilization and embryo transfer(IVF-ET), Intracytoplasmic sperm injection(ICSI), Day 2/Day 3 embryo transfer(ET), Multiple pregnancy, Cumulative embryo score(CES)