Clusterin expression and paclitaxel resistance in cervical cancer cell lines.

Shin, Jae Eun; Shin, Eun Young; Kim, Yong Wook; Kim, Dae Hoon; Park, Dong Choon

Original Article

Korean Journal of Obstetrics & Gynecology 2006;49(5):1060-1064.
Published online May 1, 2006.

Clusterin expression and paclitaxel resistance in cervical cancer cell lines.

Jae Eun Shin, Eun Young Shin, Yong Wook Kim, Dae Hoon Kim, Dong Choon Park

¹Department of Obstetrics and Gynecology, The Catholic University of Korea. dcpark@catholic.ac.kr
²Research Institute of Medical Science of Saint Vincent Hospital, The Catholic University of Korea.

Abstract

OBJECTIVE
The purpose of this study was to evaluate whether clusterin expression is significantly correlated with paclitaxel resistance in cervical cancer cell lines. METHODS: The expressions of clusterin in cervical cancer cell lines were validated with western blotting. And subsequently, the correlation between clusterin expression levels and the IC50 of paclitaxel in several cervical cancer cell lines was evaluated. After transfection of clusterin siRNA into HeLaS3, which expressed high level of clusterin, their effect on chemo-resistance was evaluated by XTT assay. RESULTS: Clusterin expression levels were correlated with paclitaxel resistance in cervical cancer cell lines. And after transfection of clusterin siRNA into HeLa-S3 significantly decreased paclitaxel resistance (P<0.05). CONCLUSION: These findings suggest that clusterin expression may correlate with paclitaxel resistance.

Key Words: Clusterin, Cervical cancer, Paclitaxel, Drug resistance