| Comparison of cell growth suppression in SiHa cervical carcinoma cell line by human papillomavirus type 16 E6/E7 siRNAs. |
|
Sae Hyun Park, Byung Joon Park, Yong Wook Kim, Duck Yeong Ro, Tae Eung Kim, Jae Keun Jung, Su Mi Bae, Woong Shick Ahn |
1Department of Obstetrics and Gynecology, College of Medicine, The Catholic University of Korea, Seoul, Korea. ahnlab1@catholic.ac.kr
2Catholic Research Institutes of Medical Science, The Catholic University of Korea, Seoul, Korea. |
|
|
| Abstract |
OBJECTIVE
Human cervical cancer is caused by the high-risk types of human papillomavirus (HPV) such as HPV16, which possess the E6 and E7 oncogenes, whose expressions are a prerequisite for cancer development. We performed this study to compare the efficacy of antitumor activity by HPV siRNA which silences only E6 or both E6/E7. METHODS: We transfected siRNA 377 (HPV16 E6 siRNA), siRNA 3 (HPV16 E6 siRNA), and siRNA 198 (HPV16 E7 siRNA) into SiHa cell line (siRNA 377 silences only E6, and siRNA 3 and siRNA 198 silence both E6 and E7). We experimented cell counts and morphologic changes 24 and 48 hours after transfection and expressions of HPV16 E6/E7 mRNA by RT-PCR. RESULTS: siRNA 377, siRNA 3, and siRNA 198 suppressed the cell growth. siRNA 3 and siRNA 198 were more potent than siRNA 377 in cell growth suppression. siRNA 377 knocked down the expression of E6 mRNA, and both siRNA 3 and siRNA 198 knocked down the expression of E6/E7 mRNA. CONCLUSION: Our results suggest that simultaneous suppression of E6 and E7 was more potent than E6-specific suppression in cancer cell growth. |
| Key Words:
Cervical cancer, Human papillomavirus, E6, E7, siRNA |
|
PDF Links
Full text via DOI
Download Citation
