Immunohisochemical study of BRCA1, BRCA2, and poly (ADP-ribose) polymerase-1 in ovarian tumors.

Choi, Se Kyung; Cho, Tae Il; Lee, Tae Jin

doi:2012.55.1.8

Obstet Gynecol Sci > Volume 55(1); 2012 > Article

Original Article

Korean Journal of Obstetrics & Gynecology 2012;55(1):8-16.
DOI: https://doi.org/10.5468/KJOG.2012.55.1.8 Published online January 1, 2012.

Immunohisochemical study of BRCA1, BRCA2, and poly (ADP-ribose) polymerase-1 in ovarian tumors.

Se Kyung Choi, Tae Il Cho, Tae Jin Lee

¹Department of Obstetrics and Gynecology, Sungae Hospital, Seoul, Korea.
²Department of Pathology, Chung-Ang University College of Medicine, Seoul, Korea. taejlee@cau.ac.kr

Abstract

OBJECTIVE
BRCA1 and BRCA2 are putative tumor suppressor gene responsible for hereditary ovarian cancer syndrome. Both BRCA1 and BRCA2 are involved in maintaining genome integrity in DNA repair. In the complex DNA repair related proteins such as poly (ADP-ribose) polymerase-1 (PARP1) have been less studied in ovarian cancer. The aim of our work is to analysis the protein expression of BRCA1, BRCA2 and PARP1 in ovarian tumors including benign, borderline, malignant tumors. METHODS: We selected 10 cases of normal tissue, 24 cases of borderline tumors, 40 cases of benign tumors, and 52 cases of carcinomas in ovary. Immunohistochemistry for BRCA1, BRCA2, and PARP1 was performed on paraffin sections. RESULTS: Negative expression of BRCA1 and BRCA2 was found in 12.5% and 22.5% of benign tumors, 25.0% and 33.3% of borderline tumors, and 36.5% and 40.4% of carcinomas, respectively. Expression of PARP1 was detected in 22.5% of benign tumors, 58.3% of borderline tumors, and 69.2% of carcinomas. Negative expression of BRCA1 with BRCA2 correlated with the expression of PARP1. CONCLUSION: Negative expression of BRCA1 with BRCA2 and PARP1 expression may play an important role in the development of ovarian tumor.

Key Words: BRCA1, BRCA2, PARP1, Ovarian tumor